Digoxin uptake, storage and imprinting

 

 

 

 

(Darvas, Zs., Swydan, R., Csaba, G., László, V., Nagy, SU. Imprinting, digoxin uptake and storage in Tetrahymena. Acta Physiol. Hung. 65, 125-128, 1985)

 

                

Digoxin consumption and release of Tetrahymena

 

                        D/C                                       C/D                                    D/D

            (release into the medium)     uptake from the medium     uptake from the medium

                        Days                                     Days                                 Days

 

 

1

3

9

15

1

3

9

15

1

3

9

15

30’

2.02

2.97

-

-

3.04

5.15

7.7

9.77

4.5

6.07

7.75

6.43

1 h

2.97

1.27

-

-

1.14

3.74

7.25

7.53

4.95

4.4

7.79

7.2

4 h

1.66

1.65

-

-

2.74

3.02

6.46

8.10

5.33

5.29

4.95

5.18

24 h

1.8

1.8

-

-

2.89

3.74

6.46

6.07

4.09

3.6

6.61

4.43

Mean

1.87

1.92

-

-

2.49

3.91

6.96

6.61

4.71

4.84

6.77

5.78

 

Determination by radioimmunoassay (ng/ml). In the control (C/C) group 2.4 ng/ml digoxin was detected in the culture medium. Group D/C= cultivation was started in 150 ml nutritive fluid containing 12.5 mg/ml digoxin. After 24 h the sample was washed thoroughly, centrifuged and transferred into 10 ml of normal culture medium for 1, 3, 9 or 15 days. C/D= cultivation in 10 ml nutritive fluid containing 5 ng/ml of digoxin. D/D= cultures treated as D/C, then further dose of digoxin was added at time points described. Volume of nutritive fluid = 10 ml, concentration of digoxin 5 ng/ml.

 

Conclusions:

1.Tetrahymena contains digoxin-like material,

2. Tetrahymena secretes digoxin into the culture medium,

3. Digoxin uptake and imprinting are also present, and re-administration of digoxin enhances  digoxin consumption.