D/C
C/D
D/D
(release into the
medium) uptake from the medium uptake from the medium
Days Days Days
|
|
1 |
3 |
9 |
15 |
1 |
3 |
9 |
15 |
1 |
3 |
9 |
15 |
|
30’ |
2.02 |
2.97 |
- |
- |
3.04 |
5.15 |
7.7 |
9.77 |
4.5 |
6.07 |
7.75 |
6.43 |
|
1 h |
2.97 |
1.27 |
- |
- |
1.14 |
3.74 |
7.25 |
7.53 |
4.95 |
4.4 |
7.79 |
7.2 |
|
4 h |
1.66 |
1.65 |
- |
- |
2.74 |
3.02 |
6.46 |
8.10 |
5.33 |
5.29 |
4.95 |
5.18 |
|
24 h |
1.8 |
1.8 |
- |
- |
2.89 |
3.74 |
6.46 |
6.07 |
4.09 |
3.6 |
6.61 |
4.43 |
|
Mean |
1.87 |
1.92 |
- |
- |
2.49 |
3.91 |
6.96 |
6.61 |
4.71 |
4.84 |
6.77 |
5.78 |
Determination by radioimmunoassay (ng/ml). In the control (C/C) group 2.4 ng/ml digoxin was detected in the culture medium. Group D/C= cultivation was started in 150 ml nutritive fluid containing 12.5 mg/ml digoxin. After 24 h the sample was washed thoroughly, centrifuged and transferred into 10 ml of normal culture medium for 1, 3, 9 or 15 days. C/D= cultivation in 10 ml nutritive fluid containing 5 ng/ml of digoxin. D/D= cultures treated as D/C, then further dose of digoxin was added at time points described. Volume of nutritive fluid = 10 ml, concentration of digoxin 5 ng/ml.
Conclusions:
1.Tetrahymena contains digoxin-like
material,
2. Tetrahymena secretes digoxin into the culture medium,
3. Digoxin uptake and imprinting are also present, and re-administration of digoxin enhances digoxin consumption.