Effect of
polypeptides and imprinting of
Tetrahymena.
a.
effect of polypeptides on growth rate
|
Time |
insulin |
Glucag |
Protam |
TSH |
PMSG |
ACTH |
BSA |
Control |
|
24 h |
17.6 |
18.4 |
17.1 |
14.5 |
15.6 |
14.4 |
16.8 |
12.8 |
|
24 h re-expos. |
17.4 |
18.2 |
17.6 |
17.6 |
16.8 |
15.2 |
16.4 |
13.1 |
|
7 days |
15.1 |
15.4 |
14.7 |
14.6 |
14.0 |
13.8 |
13.5 |
12.25 |
|
7 days reexp. |
18.3 |
19.2 |
17.6 |
16.4 |
15.0 |
15.1 |
15.6 |
12.6 |
Glucag=glucagon, protam=protamine,
TSH=thyrotropic hormone, PMSG=pragnant mare serum gonadotropin = chorionic
gonadotropin, ACTH=adrenocorticotropic hormone, BSA=bovine serum albumin. Mean
growth rates (mean number of cells in twenty clones of each group).
b.
re-exposition with the same or foreign polypeptide,
effect on binding
|
BSA-FITC-BSA |
BSA- FITC-BSA |
BSA-FITC-Protami-ne |
BSA-FITC-Protami-ne |
Protamine-FITC-Protamine |
Protamine-FITC-Protamine |
Protamine-FITC-BSA |
Protamine-FITC-BSA |
|
1 day |
7 days |
1 day |
7 days |
1 day |
7 days |
1 day |
7 days |
|
153.55 |
120.58 |
163.05 |
132.12 |
178.43 |
144.21 |
134.93 |
133.45 |
The figures represent percents
relative to control as 100%. Significance to control p<0.01 in each case.
Conclusions:
1. Polypeptide
hormones and non-hormone polypeptides stimulated
the growth of Tetrahymena
2. Re-exposure after 24 h accounted for a greater growth stimulation in case of TSH and PMSG, and after 7 days in all
3. Non-hormone polypeptides also have
imprinting-potential and cross imprint each other
4. A single treatment with a polypeptide stimulate the growth of
Tetrahymena at least for as long as 1 week