Table 30

Digoxin and ouabain imprinting

(Csaba, G., Swydan, R., Darvas, Zs. Digoxin effect and imprinting in the unicellular Tetrahymena. Acta Physiol. Hung. 67, 271-275, 1985)

a. effect of digoxin treatment and reexposure on growth

	Group		C/C		C/D		D/C		D/D
Mean+/-s.d.		1.00		1.09+/-0.04		1.06+/-0.15		0,85+/-0.06

C=control (not treated); D=digoxin-treated.

Significance to control:C/D and D/D p<0.01

b. effect of ouabain treatment and reexposure on growth

	Group		C/C		C/O		O/C		O/O
Mean+/-Sx		1.00		0.89+/-0.03		1.35+/-0.15		1.34+/-0.27

C=control (not treated); O=ouabain treated.

Significance to control:C/O and O/C p<0.01; O/O p<0.05

c. effect of digoxin (D) or ouabain (O) imprinting to the binding and incorporation of

³H-labelled glycosides.

Group	D/C	D/D	O/C	O/O
Grains/cell	8.5	18.0	8.5	19.35

C=control (not treated); D=digoxin treated; O=ouabain treated.

Significance to the appropriate control: p<0.01

Conclusions:

1. Digoxin treatment increased, whereas ouabain treatment decreased the growth rate of Tetrahymena.
2. Double digoxin treatment decreased the growth.
3. Ouabain treatment increased the growth rate of progeny generations and this was not influenced by ouabain reexposure.
4. Both digoxin and ouabain imprinting enhanced the later binding (incorporation) of the appropriate glycoside.